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Your Position :Home->Past Journals Catalog->2015年52 No.3

Identification and expression profiles of Cnaphalocrocis medinalis carboxylesterase genes
Author of the article:LIU Su** FENG Ming-Feng HE Meng-Zhu
Author's Workplace:College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Key Words:Cnaphalocrocis medinalis, carboxylesterase, phylogenetic analysis, expression profiles
Abstract:  [Objectives]  To identify carboxylesterase genes (CarEs) in Cnaphalocrocis medinalis and determine their expression profiles in various adult tissues. [Methods]  C. medinalis transcriptome datasets were searched to identify CarEs. Bioinformatic software was used to analyze the identified CarE sequences, and a real-time quantitative PCR assay was performed to investigate their relative expression levels. [Results]  A total of 15 CarEs were identified from the C. medinalis transcriptome datasets and named CmCarE1-CmCarE15. Of these, CmCarE12 lacked the 3¢-region whereas the other 14 sequences contained complete open reading frames (ORFs). With the exception of CmCarE11, protein encoded by these CmCarE genes showed features typical of CarEs, including a conserved pentapeptide, catalytic triad, and oxyanion hole. Phylogenetic analysis showed that the 15 CmCarEs fell into different clades; CmCarE13, CmCarE14 and CmCarE15 fell into the “intracellular catalytic class”, whereas the other 12 CmCarEs fell into the “secreted catalytic class”. CmCarE1, CmCarE2, CmCarE3, CmCarE7, CmCarE8, CmCarE10 and CmCarE13 were specifically expressed in the adult abdomen, whereas CmCarE9 was specifically expressed in male and female antennae. Other genes were not tissue-specific. [Conclusion]  CmCarE1, CmCarE2, CmCarE3, CmCarE7, CmCarE8, CmCarE10 and CmCarE13 might possibly be involved in the metabolism of endobiotic and xenobiotic compounds, whereas CmCarE9 might be involved in the degradation of odorant molecules.
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