DNA barcoding identification kit technology for Tetropium species
Author of the article:CHEN Meng-Yi1, 2** XU Mei3 QIAN Lu3 AN Yu-Lin3 WANG Yan4 HAO De-Jun1, 2***
Author's Workplace：1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China; 3. Jiangsu Entry-Exit Inspection and Quarantine Bureau , Nanjing 210001 China; 4. Forest Station of Shanghai, Shanghai 200072, China
Key Words：Tetropium, DNA barcode, COⅠ gene, molecular identification
Abstract： [Objectives] To rapidly and accurately identify Tetropium spp., which are invasive pests commonly intercepted at quarantine ports in shipments of wood, and improve on current, mainly morphologically-based, methods of identifying these pests. [Methods] A DNA barcode detection kit was developed for Tetropium spp. based on the mitochondrial COⅠ gene. DNA extraction methods followed those of the Canadian Plant Development DNA barcode Center. [Results] Nine base sites were obtained from Tetropium spp. [Conclusion] Analysis of different genetic characteristics among Tetropium spp. allowed nine species to be identified. The newly developed DNA barcoding identification kit provides a new way of identifying this pest at ports of entry and exit.