[Objectives]  To find which method of extracting and preserving the DNA of adult Pseudococcidae specimens produced the best quality genomic DNA. [Methods]  Genomic Phenacoccus solenopsis DNA from either fresh living adult specimens, or those that had been preserved for over 1 year in 100% ethanol at﹣20℃, or 100% ethanol at 4℃, was extracted using four methods; CTAB, SDS, GenMagBio kit and GeneJET kit, and the quality and purity of the extracted genomic DNA compared. [Results]  The best quality genomic DNA was obtained from fresh living specimens. Samples that had been stored in 100% ethanol at ﹣20℃ or 100% ethanol at 4℃ showed evidence of DNA degradation, there was no significant difference in the quality of the DNA obtained from these two storage methods. For fresh samples, the CTAB method produced the best quality DNA, the GenMagBio kit and SDS were produced the next best, and the GeneJET kit produced the worst. However, for samples that had been preserved in 100% ethanol, the GenMagBio kit was the best extraction method. [Conclusion]  Storage in 100% ethanol at ﹣20℃ is suitable for the long term preservation of adult Pseudococcidae specimens and DNA samples obtained from such specimens are suitable for PCR amplification and sequencing. The CTAB method is best for fresh adult specimens but the GenMagBio kit is the best extraction method for specimens that have been stored in ethanol for a long period, especially if there has been serious DNA degradation.