Electrical penetration graph (EPG) is used to record the characteristics of electrical signal variation caused by sucking insects when their stylet pierces a host plant’s tissues. This can establish the correlation between an insect’s feeding behavior and EPG waveform types. In recent years, EPG has been widely used to study insect feeding behavior and virus transmission, and the mechanisms of host plants’ resistance to sucking insects. During EPG experiments, the method using to fix insect subjects is a pivotal skill that can affect the connectivity between the insect and the EPG electrodes. The small brown planthopper (SBPH), Laodelphax striatellus (Fallén) was used as a test insect to study and compare three commonly used fixation techniques (i.e., the negative pressure, anesthetization, and freezing, methods). On the basis of the results, these fixation techniques were ranked, in descending order; negative pressure method, anesthetization, freezing methods.