Homology modeling of the odorant binding protein of the pineshoot beetle, Tomicus yunnanensis
Author of the article:WANG Yu1** SUN Hao2 ZHU Jia-Ying1 ZHAO Ning1 YANG Bin1***
Author's Workplace：1. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China; 2.College of Science, Southwest Forestry University, Kunming 650224, China
Key Words：odorant binding protein, homology modeling, three-dimensional structure, Tomicus yunnanensis
Abstract： [Objectives] This study aims to provide the basis for revealing the mechanism of the odorant binding protein of the pine shoot beetle, Tomicus yunnanensis involved in olfactory reorganization [Methods] The three dimensional structure of T. yunnanensis’s OBP was constructed using the homology modeling method and the reliability of the model was evaluated by Procheck, Verify_3D and ERRATERRAT. [Results] Analysis of the optimized structure found that the OBP of T. yunnanensis contains 6 conservative cysteine residues in its sequence. Evaluation of the model by Procheck, Verify_3D and ERRATERRAT indicated that it is reasonable and reliable. [Conclusion] The results showed that it is possible to predict the structure of a protein that has a known sequence with unknown spatial structure based on known structural information of its homologous proteins. The results provide a foundation for further research on the functional structure domain of T. yunnanensis OBP.