[Objectives]  The two-spotted spider mite Tetranychus urticae Koch is one of the most economically important pests of a wide range of crops and pastoral grasses worldwide. To clarify the biological characteristics of T. urticae, the effects of different clover species on its development and fecundity were investigated. [Methods]  Three treatment groups of two-spotted spider mites were fed either red clover, white clover, or alsike clover, under laboratory conditions at a constant temperature (25±1)℃. [Results]  The three clover species had significant effects on the developmental time, adult female longevity and fecundity (P<0.05). The developmental time and pre-oviposition period of mites fed on alsike clover were longer than in those fed on red or white clover, and adult female longevity and the duration of oviposition were markedly shorter. Mites fed on red clover laid the highest average number of eggs per female (150.87), followed by those fed on white clover (139.43), whereas those fed on alsike clover laid the lowest (86.95). The survival curves of all three treatment groups were Deevey type I. Survival was highest on red clover, followed by white clover, and lowest in mites fed on alsike clover. The net reproductive rate (R0), intrinsic rate of increase (rm) and finite rate of increase (λ) were highest on red clover and lowest on alsike clover, and mean generation time (T) and population doubling time (Dt) were longest on alsike clover and lowest on red clover. [Conclusion]  Clover species had a strong effect on the development and fecundity of the two-spotted spider mite which had higher fitness on red and white clover than on alsike clover