Techniques for assaying detoxification enzyme activity in Locusta migratoria
Author of the article:DONG Wei** WU Hai-Hua ZHANG Jian-Zhen WANG Ze-Shi MA En-Bo*
Author's Workplace:Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China
Key Words:Locusta migratoria, detoxification enzymes, non-specificesterases, glutathione S-transferases, cytochrome P450 monooxygenases
Abstract:
Locusta migratoria (L.) is a major agricultural pest and metabolic resistance is one of the most important mechanisms by which this species develops resistance to insecticides. The detoxification enzymes, which are associated with metabolic resistance, include nonspecific esterase (ESTs), glutathione S-transferases (GSTs) and cytochrome P450 monooxygenases (P450s). Assaying detoxification enzyme activity is an important way to study pesticide metabolism in L. migratoria. This paper describes in detail how to analyze the detoxification enzymes activity in L. migratoria, and provides a reference for this in other insects.