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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
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Your Position :Home->Past Journals Catalog->2016年53 No.3

Expression of ABCC1-ABCC5 mRNA in the diamondback mothsubject to chlorantraniliprole stress
Author of the article:WANG Meng-Lun1** LIANG Pei2 JIN Dao-Chao1***
Author's Workplace:1. Institute of Entomology, Guizhou University, Guiyang 550025, China; 2. Department of Entomology, China Agricultural University, Beijing 100193, China
Key Words:Plutella xylostella, chlorantraniliprole, RT-qPCR, ABCC1-ABCC5
Abstract:

 [Objectives]  To elucidate the relationship between chlorantraniliprole and the ABC transporter (ATP-binding cassette transporter) in the diamondback moth, Plutella xylostella. [Methods]  ABCC1-ABCC5 mRNA expression in different strains of the diamondback moth, and the LC50 of a susceptible strain to chlorantraniliprole, were determined using real-time quantitative RT-PCR, after which the expression of ABCC3, ABCC4 and ABCC5 in different developmental stages and tissues of 4th instar larvae was measured. [Results]  All 5 genes were up-regulated in the resistant strain (CHR) where their expression was significantly higher than in the susceptible strain (CHS). Expression of ABCC3, ABCC4 and ABCC5 were 2.16, 2.81, and 1.85 times higher in the CHS following treatment with the LC50 dose of chlorantraniliprole than in the untreated control, but ABCC1 and ABCC2 were down-regulated and their expression was only 65.8% and 37.2% that of the control, respectively. The expression of ABCC3 mRNA in the larval stage and in male adults was significantly higher than in other developmental stages. There was no obvious difference in the expression of ABCC4 mRNA among the developmental stages examined. The expression of ABCC5 mRNA in third instar larvae was significantly higher than in other developmental stages. In 4th instar larvae, the expression of ABCC3, ABCC4 and ABCC5 mRNA in the midgut was significantly higher than in other tissues, and the expression of ABCC4 mRNA was significantly higher than that of ABCC3 and ABCC5 in the same tissue. [Conclusion]  The results indicate that ABCC3, ABCC4 and ABCC5 may be involved in the detoxification of chlorantraniliprole in the diamondback moth. Among three genes, ABCC4 gene may be the most important in detoxification. These results provide a basis for further study of the relationship between ABCC1-ABCC5 and the metabolic mechanisms involved in the detoxification of chlorantraniliprole in the diamondback moth.

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