[Objectives] To clone the HaPOD peroxidase gene in Helicoverpa armigera and analyze its function, including its temporal and spatial expression profiles, and determine its expression after injecting H₂O₂, exposure to extreme temperature and HaNPV infection. ［Methods］Full-length HaPOD cDNA was amplified using RT-PCR based on the transcriptome sequencing results for H. armigera. The gene and amino acid sequences of HaPOD were analyzed with several kinds of bioinformatics software. The spatio-temporal expression profiles of the HaPOD gene, and its expression patterns after 4 kinds of stress treatment (injection with H₂O₂, infection by HaNPV, and exposure to hot and cold temperatures) were determined by qRT-PCR. ［Results］Sequence analysis revealed that the ORF of the HaPOD gene was 1 332 bp and encoded 443 amino acids containing a peroxinectin sequence. The qRT-PCR results showed that the transcription level of HaPOD genes was low in larvae < 5 days of age, but increased to a maximum on the fifth day of the pupal stage. Spatial expression profiling indicates that the HaPOD gene was mainly expressed in the heads of larvae and in the wings of adults. Transcription of HaPOD markedly increased following injection with H₂O₂, HaNPV, or exposure to high temperature (35℃), but decreased following exposure to cold (4℃).［Conclusion］ The ORF of the HaPOD gene was successfully cloned and analyzed. Its expression was significantly upregulated by injection with H₂O₂, infection by HaNPV and exposure to high temperature, but was downregulated by cold temperature. These results provide a foundation for further research on the mechanisms involved in maintaining the redox balance and protection against oxidative damage.