Molecular identification of octopamine receptor 2 in Helicoverpa armigera (Lepidoptera: Noctuidae)
Author of the article:WU Feng-Ming1, 2** WU Peng-Xiang1, 2 WANG Xuan1 YAN Shuo3 LI Zhen1 ZHANG Qing-Wen1 LIU Xiao-X
Author's Workplace:1. China Agricultural University, Beijing 100193, China; 2. Institute of Zoology, Chinese Academy of Sciences, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Beijing 100101, China; 3. National Agricultural Technology Extension and Service Center, Beijing 100125, China
Key Words:Helicoverpa armigera, octopamine receptor 2, moulting hormone, RNA interference
Abstract:
[Objectives] To investigate the expression and hormone regulation of the octopamine receptor 2 gene (HaOA2B2) in the cotton bollworm, Helicoverpa armigera, and thereby clarify the role of HaOA2B2 in transcription regulation and provide a theoretical basis for the development of new pesticides. [Methods] QRT-PCR, hormone treatment, and RNAi mediated ecdysone nuclear receptor silencing, were used to investigate the expression, and hormonal regulation, of HaOA2B2 in different developmental stages and tissues of the cotton bollworm. [Results] The ORF length of HaOA2B2 is 1 191 bp, encoding 396 amino acids. HaOA2B2 shares up to 89% amino acid sequence similarity with silkworm (Bombyx mori) octopamine receptors. HaOA2B2 was expressed in all developmental stages and was most highly expressed on the 3rd day of the 5th instar. Expression was higher in the head than in other organs or tissues. Ecdysone can promote HaOA2B2 receptor gene expression. [Conclusion] The sequence characteristics and expression profiles of the HaOA2B2 receptor gene were determined, plus the effect of ecdysone on the gene’s expression. These results provide a theoretical basis for future research on the role of the HaOA2B2 receptor gene in transcription regulation and the development of new pesticides.