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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2016年53 No.5

Cloning and expression analysis of ribosomal protein S8 gene, NlRPS8 in the brown planthopper (Nilaparvata lugens)
Author of the article:ZHU Jia-Jun** HAO Pei-Ying*** LU Chao-Feng FENG Ya-Lin YU Xiao-Ping***
Author's Workplace:Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Key Words: Nilaparvata lugens, NlRPS8 gene, RT-qRCR, virulence variation
Abstract:

[Objectives]  Ribosomal proteins play very important roles in the growth and development of organisms. This study aimed to determine the expression pattern and function of a ribosomal protein S8 gene, NlRPS8 (GenBank No. KU341337) in the rice brown planthopper, Nilaparvata lugens. [Methods]  The full length cDNA of NlRPS8 was amplified from N. lugens by RT-PCR and rapid-amplification of cDNA ends using RNA-seq analysis of transcriptome and gene expression profile information. [Results]  The results show that NlRPS8 has an open reading frame of 627 bp encoding 208 amino acid residues. A phylogenetic tree based on variation in the NlRPS8 gene shows that N. lugens is most closely related to Maconellicoccus hirsutus. qRT-RCR analysis revealed that the mRNA level was highest in gravid females but relatively low in adult males, newly eclosed female adults, and nymphs. qRT-RCR analysis of the head, thorax, fat body, ovaries, and mid gut, revealed that NlRPS8 was most highly expressed in the ovaries. The relative expression levels of NlRPS8 in the resistant rice varieties ASD7 and RHT were 1.99 and 2.14 times, respectively, that in the susceptible rice variety TN1. The relative expression of NlRPS8 in a treatment group provided with water only was 1.6 times that of a group fed TN1 rice. [Conclusion]  This study provides new clues for further research on the function of RPS8 in N. lugens development and its adaptation to resistant rice varieties.

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