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Your Position :Home->Past Journals Catalog->2016年53 No.6

Behavioral responses of female Bradysiaod oriphaga Yang et Zhangto volatiles of conspecific larvae, pupae and eggs
Author of the article:ZHANG Zhi-Jun1** LI Wen-Xiang2 HE Min3 ZHU Xiao-Dan3 LIN Wen-Cai1 LI Xiao-Wei1 LI Wei-Di1 ZHAN
Author's Workplace:1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. HebeiNorth University, Zhangjiakou 075000, China; 3. Institute of Plant Protection and Environment Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China
Key Words:Bradysia odoriphaga, Y-tube olfactometer, behavioral response, volatile
Abstract:

 [Objectives]  To determine the preferences of adult female Bradysiaodoriphaga Yang et Zhang to volatile compounds emitted by conspecific larvae, pupae and eggs, in order to find more effective lures for trapping, and establishing green control technology for this pest. [Methods]  The behavioral responses of female B. odoriphaga Yang et Zhang to volatiles emitted by conspecific 2nd and 4th instar larvae, pupae and eggs were investigated using a Y-tube olfactometer. Volatile substances that attracted female adults were then qualitatively and quantitatively analyzed using solid micro-extraction (SPME) and a Gas Chromatography-Mass Spectrometer (GC-MS). The relative attractiveness of different chemical compounds, and ratios of compounds, to adult females were then determined using a Y-tube olfactometer. [Results]  Both virgin and mated adult females were significantly attracted to the volatiles emitted by 4th instar larvae, and were not attracted to those emitted by 2nd instar larvae, pupae or eggs. Dipropyl disulfide and 2, 2-dimethyl- 1, 3-dithiane were two main components of larval volatiles and the amount of the former was 10 fold that of the latter. Mated adult females were clearly attracted by 50mg of dipropyl disulfide, and also by 50mg or 5mg of 2, 2-dimethyl- 1, 3-dithiane, but not significantly. Adult females were strongly attracted by 1︰1 (50 mg︰50 mg) and 10︰1(50 mg︰5mg) ratios of dipropyl disulfide : 2, 2-dimethyl- 1,3-dithiane. The 10︰1 (50 mg︰5 mg) ratio of dipropyl disulfide: 2, 2-dimethyl- 1, 3-dithiane was significantly more attractive than 50 mg of dipropyl disulfide. [Conclusion]  Female B. odoriphaga significantly prefer the odor of 4th instar larvae and are attracted by dipropyldisulfide et al.disulfides, the attractiveness of which can be increased by the addition of 2, 2-dimethyl- 1, 3-dithiane.

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