Neonicotinoid insecticide resistance in Bradysia odoriphaga Yang et Zhang
Author of the article:CHEN Cheng-Yu** WANG Cui-Cui GUO Jing-Jing XU Guo-ShengSHI Xue-Yan*** SONG Dun-Lun
Author's Workplace：College of Plant Protection, China Agricultural University, Beijing 100193, China
Key Words：Bradysia odoriphaga, neonicotinoid insecticides, resistance monitoring, resistance mechanism
Abstract：[Objectives] To clarify the level of resistance of Bradysia odoriphaga to neonicotinoid insecticides and the mechanism responsible for this resistance. [Methods] Larvae of B.odoriphaga were collected from different regions of China and their sensitivity to three neonicotinoids; imidacloprid, thiamethoxam and clothianidin, examined. In addition, the P450s-related resistance mechanism to neonicotinoids was explored. [Results and Conclusion] Four field populations of B. odoriphaga had different resistance levels to the three neonicotinoids tested. Among these populations, the TS population had the highest resistance to all three neonicotinoids. ECOD activity of the TS population was (3.89±0.31)pmol/(mg·pro·min), higher than that of the SS population. Moreover, the synergic ratio of PBO to imidacloprid toxicity in the TS population was 2.64, which was higher than that of the susceptible population to PBO (1.08). This suggests that elevated P450s activity is related to imidacloprid resistance in B. odoriphaga larvae.