[Objectives]  Myiasis is a widely distributed kind of parasitic disease mainly caused by fly larvae. Psychoda alternata is one of the most common species of psychodidae that can trigger human myiasis. Determining the complete mitochondrial genome of P. alternata is valuable for general molecular and evolutionary studies on the Diptera, as well as for providing a theoretical basis for the prevention and treatment of myiasis. [Methods]  DNA sequencing and cloning technology were used to obtain the complete mitochondrial genome sequence of P. alternata. [Results]  The complete mitochondrial genome contained 15 908 nucleotides, consisting of protein coding genes, tRNA genes, rRNA genes, and a non-coding A+T-rich region. Two strands, the J and N strands, transcribed 37 genes. Genomic base composition was A 39.6%, T 40.3%, C 11.8%, G 8.3%, and A+T content comprised 79.9%. [Conclusion]  The genetic organization of the P. alternata mitochondrial genome sequence is consistent with that of typical insect mtDNA. The J strand transcribes 9 protein genes whereas the other 4 genes are distributed on the N strand. The phenomenon of A+T composition asymmetry was not obvious. Phylogenetic analyses based on 13 protein-coding genes from mitochondrial genomes in 26 dipteran species demonstrates that the Psychodidae and Tanyderidae are sister taxa.