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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2018年55 No.1

Impact of the rice stripe virus (RSV) on the biological, physiological and biochemical characteristics of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae)
Author of the article:HE Kang1, 2** GUO Jin-Meng1 LI Fei2 LIN Ke-Jian1*** WANG Gui-Rong1
Author's Workplace:1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
Key Words: Laodelphax striatellus, rice stripe virus (RSV), longevity, ovary development,enzyme activity
Abstract:

 [Objectives]  To investigate how infection with the rice stripe virus affects biological characteristics of Laodelphax striatellus Fallén, including development, ovarian development and enzyme activity. [Methods]  The single-tube rearing method was used to measure the duration of the developmental period, nymph period, and adult longevity, of the small planthopper (SBPH). Enzyme activity assays were used to compare the activity of five physiological protection and detoxification enzymes in nymphs and adults, and the effect of RSV infection on ovarian development was studied by counting the number of eggs in infected and uninfected females. [Results]  The uninfected female and male nymph periods were (16.30 ± 0.33 ) days and (15.62 ± 0.21) days, respectively, compared to (19.08 ± 0.43) days and (18.50 ± 0.58) days, respectively for infected female and male nymphs. A paired t-test indicates that RSV infection significantly prolonged the duration of the nymph period (P < 0.001). The longevity of uninfected female and male adults was (10.74 ± 0.81) days and (14.46 ± 1.34) days, respectively, compared to (9.09 ± 1.27) days and (13.55 ± 2.38) days for infected female and male adults. Results of a paired t-test indicate that RSV infection did not significantly affect adult development (P > 0.05). Ovarian development and oogenesis were not significantly affected by infection with RSV. Activity assays for protective enzymes, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and detoxification enzyme glutathione S-transferase (GST) and acetylcholinesterase (AChE), were carried out on infected and uninfected 3rd instar nymphs at 12, 24 and 120 hours. The results indicate that RSV infection does not significantly affect enzyme activity other than causing slight variation in trends of enzyme activity over time. [Conclusion]  RSV affects the development of SBPH nymphs, but not other biological characteristics of the SBPH, such as immune system and ovarian development. In addition to prolonging the duration of the nymph period and slightly affecting variation in the trend of enzyme activity, there is no obvious reciprocal and mutually beneficial relationship between the SBPH and the RSV.

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