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Your Position :Home->Past Journals Catalog->2018年55 No.2

The effect of elevated atmospheric CO2 concentration on gene expression of peach aphid fed on different genotypes of Arabidopsis
Author of the article:GU Li-Yuan1, 2** LIU Zhi-Yuan2 GUO Hui-Juan2 GE Feng2 ZHANG Chao1*** SUN Yu-Cheng2***
Author's Workplace:1. College of Life Science, Hebei University, Baoding 071002, China; 2. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Key Words: peach aphid, CO2 concentration, Arabidopsis, salicylic acid
Abstract:

Global atmospheric CO2 concentration which has been increasing at an accelerating rate attracted widespread attention. Most of studies were focused on the response of plant to elevated CO2, but little was known about whether elevated CO2 can change the performance of herbivorous insects. [Methods]  In this experiment, the effects of elevated CO2 were evaluated on the transcriptome changes of green peach aphid Myzus persicae fed on two Arabidopsis genotypes, a npr1 mutant (deficient in salicylic acid signaling pathway) and wild-type control (wt) by using high-throughput screening technology. [Results]  The study showed that the increase of atmospheric CO2 concentration only promoted the weight of green peach aphid fed on npr1 mutant but had little effect on aphid fed on wild type plants. In addition, the weight of aphids fed on npr1 genotype were significantly lower than those of the wild type under ambient CO2. Furthermore, we used pathway enrichment analysis to identify metabolic pathways or signal transduction pathways that were significantly enriched in DEGs (Differential Expressed Gene) relative to the whole transcriptome background of green peach aphid associated with different treatments. Among all the genes,220, 274, 661 and 536 genes were significantly enriched in DEGs when aphids responded to elevated CO2 fed on wt plants, elevated CO2 fed on npr1 plants, plant genotypes under ambient CO2, plant genotypes under elevated CO2, respectively. Among all the genes subjected to KEGG pathway annotation and enrichment analysis, 9, 16, 12 and 15 pathways were significantly enriched in DEGs when aphids responded to elevated CO2 fed on wt plants, elevated CO2 fed on npr1 plants, plant genotypes under ambient CO2, plant genotypes under elevated CO2, respectively. The common pathways significantly enriched in DEGs when aphids responded to plant genotypes under ambient CO2 and elevated CO2 fed on npr1 plants: protein processing in endoplasmic reticulum, proteasome, pyruvate metabolism and antigen processing and presentation. [Conclusion]  The effects of elevated CO2 on aphid weight exhibit host plant genotype-specific. The enhancement of elevated CO2 on the body weight of aphids associated with npr1 mainly due to the increase of aphid primary metabolism including amino acid metabolism, fatty acid metabolism as well as carbohydrate metabolism.

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