The effects of transgenic rice on the feeding behavior of the non-target aphid Rhopalosiphum maidis (Fitch) (Homoptera: Aphididae)
Author of the article:REN Shao-Peng；YANG Fan；GAO Ming-Qing；PU De-Qiang；YE Gong-Yin；SHI Min；CHEN Xue-Xin
Author's Workplace：State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China; Ningbo Academy of Agricultural Sciences, Ningbo 315040, China
Key Words： Rhopalosiphum maidis, transgenic rice, Y-tube olfactometer, EPG, feeding behavior
Abstract：
[Objectives] To assess the potential ecological risk of transgenic rice plants to a non-target pest; the corn leaf aphid, Rhopalosiphum maidis (Fitch). [Methods] The behavioral responses of aphids to volatiles of rice seedling were studied in a Y-tube olfactometer, and physiological responses of aphids to volatiles was analyzed with EPG. [Results] Non-transgenic rice lines were more attractive to aphids than transgenic rice but this difference was not significant. The response time of aphids to non-transgenic rice lines was longer than that for transgenic rice lines. Aphids preferred KMD1 to KMD2. The percentage of accumulated duration (E1), which reflects the proportion of time that aphids secreted saliva into rice phloem, was significantly higher on KMD2 than on XS11. The percentage of accumulated duration (np), which reflects the proportion of time that aphids spent moving to feeding sites, was significantly lower on 223F-S21 than on XS134. There was no significant difference in feeding behavior between G8-7 and XS110. [Conclusion] Two tested transgenic rice lines (KMD2 and 223F-S21) have no obvious adverse effects on the feeding behavior of R. maidis.