[Objectives]  Sitophilus zeamais (Motschulsky) is a global pest of a broad variety of stored foodstuffs that is found in most provinces (regions) of China. Many morphological features of S. zeamais are similar to those of S. oryzae, which makes these species difficult to distinguish reliably. Since the only diagnostic features are the male and female genitalia, it is only possible to reliably identify adults. It would be useful, therefore, to develop alternative methods that can efficiently and reliably identify other life-stages. [Methods]  Genomic DNA of S. zeamais was extracted and the ITS1、ITS2、Cytb、COⅠ、COⅡ genes amplified and sequenced via PCR using universal primers. Rapid identification on the basis of gene sequence similarity was done and a DNA barcode for the rapid identification of S. zeamais obtained. [Results]  S. zeamais specimens were identified based on morphological characteristics, and the base sequence of corresponding genes obtained and used as the DNA barcode for this species. [Conculsion]  A number of gene sequences were obtained that can be used for DNA barcoding of S. zeamais, thereby allowing the rapid and accurate identification of this pest.