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Your Position :Home->Past Journals Catalog->2018年55 No.3

Risk assessment and biochemical mechanisms responsible for resistance to pyridalyl in the diamondback moth, Plutella xylostella (Linnaeus)
Author of the article:SUN Yang;QIN Wen-Jing;HUANG Shui-Jin;QIN Hou-Guo
Author's Workplace:Institute of Plant Protection, Jiangxi Academy of Agricultural Science, Nanchang 330200, China;Institute of Plant Protection, Jiangxi Academy of Agricultural Science, Nanchang 330200, China;Institute of Plant Protection, Jiangxi Academy of Agricultural Science, Nanchang 330200, China;Institute of Plant Protection, Jiangxi Academy of Agricultural Science, Nanchang 330200, China
Key Words: [Objectives] A risk assessment and investigation of the biochemical mechanisms underlying resistance to pyridalyl in Plutella xylostella (Linnaeus) were conducted to provide a theoretical basis for the rational application of pyridalyl for controlling this pest. [Methods] The threshold character/
Abstract:

 [Objectives]  A risk assessment and investigation of the biochemical mechanisms underlying resistance to pyridalyl in Plutella xylostella (Linnaeus) were conducted to provide a theoretical basis for the rational application of pyridalyl for controlling this pest. [Methods]  The threshold character/trait analysis method from TabashnikMcGaughey was used to assess the risk of resistance. The bioassay was conducted using the leaf dipping method. Piperonyl butoxide (PBO), triphenyl phosphate (TPP), and diethyl maleate (DEM) were used in synergy bioassays. The enzyme kinetic method was used to determine the activities of glutathione-S-transferase, esterase, and mixed-functional oxidases, in both resistant and susceptible strains. [Results]  The P. xylostella strain used in this study developed 14.8-fold resistance to pyridalyl after 18 generations of selection with a realized resistance heritability (h2) of 0.155 8. Pyridalyl resistance is expected to increase 10 fold in 16.1 to 7.3 generations under selective pressures of 50% to 90%. Compared to the susceptible strain, esterase and mixed-functional oxidase activity in a pyridalyl-resistant strain significantly increased by 1.34 and 1.45-fold, respectively. In synergy bioassays, both TPP and PBO significantly increased the toxicity of pyridalyl in the resistant strain, with synergistic ratios of 121.00% and 143.00%, respectively. DME had no obvious synergistic effect. [Conclusion]  These results indicated that P. xylostella could develop significant resistance to pyridalyl. Increased esterase and mixed-functional oxidase activity may play an important role in such resistance.

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