Abstract  [Objectives]  To identify glutathione-S-transferase (GST) genes in Chilo suppressalis, determine their expression profiles in various larval tissues, and analyze their transcriptional profiles in response to chlorantraniliprole. [Methods]  The cDNA sequences of GST genes were identified from the C. suppressalis transcriptome database and their expression profiles in different larval tissues investigated with real-time quantitative PCR. Variation in GST expression levels after exposure to a sublethal dose of chlorantraniliprole was measured. [Results]  A total of 16 GSTs were identified and named CsGSTd1- CsGSTu1. These genes were classified into six families (Delta, Epsilon, Omega, Sigama, Zeta, and Theta) and an “unclassified” subgroup. CsGSTd2、CsGSTd3、CsGSTe1、CsGSTo3、CsGSTt1 were mainly expressed in the fat-body, while CsGSTe3 was enriched in the malpighian tubes. No midgut-specific genes were detected. CsGSTo2 was significantly upregulated 6 h after exposure to chlorantraniliprole whereas CsGSTd2, CsGSTd3, CsGSTe3, CsGSTo1 and CsGSTt1 were significantly downregulated. CsGSTd1, CsGSTd2, CsGSTd3, CsGSTe1, CsGSTe2, CsGSTe3, CsGSTo2, CsGSTo3, CsGSTo4 and CsGSTt1 were significantly upregulated 12 h after chlorantraniliprole exposure, but CsGSTu1 was significantly downregulated. [Conclusion]  CsGSTd1, CsGSTd2, CsGSTd3, CsGSTe1, CsGSTe2, CsGSTe3, CsGSTo2, CsGSTo3, CsGSTo4 and CsGSTt1 may play an important role in the detoxification of chlorantraniliprole.