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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2018年55 No.4

Cloning and expression of a Tachykinin-related peptide in Diaphorina citri
Author of the article:BIN Shu-Ying; KANG Cong; MO Si-Wei; SHU Ben-Shui; WU Zhong-Zhen; LIN Jin-Tian
Author's Workplace:Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Key Words: Diaphorina citri Kuwayama, Dc-TRP, gene cloning, RT-qPCR
Abstract:

[Objectives]  Tachykinin-related peptides (TRPs) are peptide neurotransmitters involved in multiple life processes. We investigated the sequence information of a tachykinin-related peptide, Dc-TRP, in Diaphorina citri Kuwayama, and further analyzed the mRNA expression levels of Dc-TRP in different developmental stages and adult tissues. [Methods] The full-length sequence of Dc-TRP was cloned from D. citri, and serial analysis and structure prediction were performed based on its nucleotide sequence. In addition, RT-qPCR was used to analyze the expression of Dc-TRP in different development stages and adult tissues. [Results]  (1) The full-length of the Dc-TRP coding sequence obtained was 600 bp and is predicted to encode 199 amino acids with a variety of conserved regions. (2) Dc-TRP was expressed in all developmental stages and adult tissues. Although there was no significant difference in expression among developmental stages, expression was significantly higher in the head than in other adult tissues. [Conclusion]  Dc-TRP was not differentially expressed in different developmental stages of D. citri but its expression was significantly higher in head than in other adult tissues. Our results provide a basis for further study of the physiological functions of Dc-TRP

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