Cloning and expression of a Tachykinin-related peptide in Diaphorina citri
Author of the article:BIN Shu-Ying; KANG Cong; MO Si-Wei; SHU Ben-Shui; WU Zhong-Zhen; LIN Jin-Tian
Author's Workplace:Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Key Words: Diaphorina citri Kuwayama, Dc-TRP, gene cloning, RT-qPCR
Abstract:
[Objectives] Tachykinin-related peptides (TRPs) are peptide
neurotransmitters involved in multiple life processes. We investigated the
sequence information of a tachykinin-related peptide, Dc-TRP, in Diaphorina citri Kuwayama, and further analyzed the mRNA expression levels of Dc-TRP in different developmental stages
and adult tissues. [Methods] The
full-length sequence of Dc-TRP was
cloned from D. citri, and serial
analysis and structure prediction were performed based on its nucleotide
sequence. In addition, RT-qPCR was used to analyze the expression of Dc-TRP in different development stages
and adult tissues. [Results] (1) The full-length of the Dc-TRP coding sequence obtained was 600
bp and is predicted to encode 199 amino acids with a variety of conserved
regions. (2) Dc-TRP was expressed in
all developmental stages and adult tissues. Although there was no significant
difference in expression among developmental stages, expression was
significantly higher in the head than in other adult tissues. [Conclusion] Dc-TRP was not differentially expressed in different developmental stages of D. citri but its expression was
significantly higher in head than in other adult tissues. Our results provide a
basis for further study of the physiological functions of Dc-TRP.