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Your Position :Home->Past Journals Catalog->2018年55 No.4

Transcriptome analysis of immune responses of Diaphorina citri Kuwayama (Homoptera:Psyllidae) infected with Beauveria bassiana
Author of the article:SONG Xiao-Bing;PENG Ai-Tian; CUI Yi-Ping;LING Jin-Feng;CHENG Bao-Ping;ZHANG Lian-H
Author's Workplace: Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China; Agricultural College of South China Agricultural University, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510640, China
Key Words: Beauveria bassiana, Diaphorina citri, infection, transcriptome
Abstract:

 [Objectives]  To identify the network of regulatory genes involved in the immune response of Diaphorina citri to infection with Beauveria bassiana, and to further investigate the immune defense mechanism of D. citri to B. bassiana. [Methods]  An Illumina high-throughput sequencing platform was used to analyze the transcriptomes of infected and uninfected D. citri 24, 48 and 72 h after treatment. Differentially expressed genes and their functions, classifications and signaling pathways were analyzed using bioinformatic tools. [Results]  138 313 non-redundant Unigenes were identified, among which N50 and N90 were 2 532 bp and 413 bp in length, respectively. The average length of all Unigenes was 1 191.26 bp. 971, 1 671, 752 differentially expressed genes (DEGs) were obtained from the transcriptome data of CK vs. S24h, CK vs. S48h, and CK vs. S72h. 405, 614 and 542 DEGs were significantly differentially enriched in 56, 83 and 60 GO terms, respectively. KEGG pathway analysis indicated that 98, 333, and 247 expressed genes were significant differentially enriched in 10, 27, and 25 metabolic pathways, respectively. [Conclusion]  Differentially expressed genes are mainly enriched in related pathways, such as energy metabolism, ion transport, transcription and translation regulation, reproduction and development regulation, and immune defence response, and most encode potential genes related to immune recognition and regulation. We identified 5 significant up-regulations of these immune-related genes. These findings provide a theoretical foundation for the study of the immune response of D. citri to entomogenous fungi. We plan to next use q-PCR to verify the expression analysis of immune-related genes to further investigate their role inthe immune response of D. citri to Beauveria bassiana.

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