Population genetic structure and genetic diversity of Adelphocoris nigritylus based on mtDNA COⅠ gene sequence variation
Author of the article:ZHANG Li-Juan; LUO Jun-Yu;ZHANG Shuai;MA Yan;WANG Chun-Yi;Lü Li-Min;ZHU Xiang-Zhen;CUI Jin-Jie
Author's Workplace:State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China
Key Words:Adelphocoris nigritylus, genetic structure, gene flow, genetic drift, population expansion
Abstract:
[Objectives] Adelphocoris
nigritylus Hsiao is a phytophagous insect that feeds on a wide
variety of plants and in recent years has damaged agricultural crops. This
study aims to explore the population genetic structure, genetic diversity, phylogeny and demographic history of this species in North China. [Methods] We applied the population genetic approach
to analyze variation in the mitochondrial DNA COⅠ gene fragment (893 bp) of 256 individuals from 10 populations. [Results] A total of 33
haplotypes were found, including one source haplotype (H5) and three high
frequency haplotypes. A Mantel test indicated no significant correlation
between genetic and geographic distance among populations. A Neutrality test
and Beast analysis indicated that all populations may have experienced
population expansion. An AMOVA and paired Fst values revealed relatively low genetic differentiation among populations, and
Migrate indicated a high level of gene flow among populations. A BI
phylogenetic tree and Network analyses indicated evidence of overall population
expansion, but also of distinct genetic clades in some individual populations and sub-regions, although
monophyletic groups have not yet developed. [Conclusion] We
speculate that human activity may be the main reason for the apparent
population expansion of A. nigritylus in
north China. The relatively low genetic differentiation among populations is
probably due to the high level of gene flow between them. The significantly
high genetic differentiation between the Langfang population and other
populations, and the relatively low genetic diversity in the Langfang
population, suggests that genetic drift may have occurred during the process of
population expansion in this population.