Phylogenetic analysis of Achroia grisella Fabricius based on the mitochondrial cytochrome c oxidase subunit I gene

Author of the article:ZHANG Yong-Hong** ZHU Feng TANG Fen-Fen SHAO Yu-Lan YANG Shuang BAI Xing-Rong***

Author's Workplace：Institute of Sericulture and Apiculture，Yunnan Academy of Agricultural Science, Mengzi 661101, China

Key Words： Achroia Grisella Fabricius; CO I gene; homoeology; evolution analysis

Abstract： [Objectives]  Achroia grisella Fabricius (small wax moth) is the main pests in the apiculture industry, in order to confirm its position of the genetic in Lepidoptera insects, cytochrome C oxidase subunit I gene (CO I) of small wax moth was cloned, and analyzed by bioinformatics analysis. [Methods]  According to the conserved sequence of mitochondrial genome mtDNA CO I gene was logged in lepidoptera insects in NCBI, the degenerate primers were designed for the study, the genome of small wax moth as a template for PCR, mtDNA CO I sequence (GenBank accession NO. MF509586) of small wax moth was cloned and sequenced. [Results]  The sequence analysis of the CO I gene coding region found that the initial code of its translation was TTG, and the base T was not terminated completely. The homology and genetic distance analysis of CO I genes showed that Galleria mellonella (greater wax moth) and small wax moth were the most homologous and the genetic distance was 88.3% and 0.117 respectively. Phylogenetic analysis found that the small wax moth and other lepidoptera insects formed a separate clade, this clade gathered in a small branch that include greater wax moth, small wax moth, the rice moth (Corcyra cephalonica) and Corcyra cephalonica. [Conclusion]  A. grisella was most closely related to G. mellonella, CO I gene was used for distinguishing the different kinds of wax moths from same genus, the research of results provided a theoretical basis for understanding the genetic background of small wax moth.