GC-EAD and behavioral responses of Anoplophora chinensis (Coleoptera: Cerambycidae) to volatiles released from Juglans mandshurica plants after different types of damage
Author of the article:MA Yan1** SHI LI-Yang1 ZHAO Yi2 XU Hua-Chao1***
Author's Workplace:(1. School of Forestry and Biotechnology of Zhejiang A&F University, Lin’an 311300, China; 2. Jujube Bureau of Dali County, Dali 715100, China)
Key Words:Anoplophora chinensis; Juglans mandshurica; plant volatiles; GC-EAD; Y-tube olfactory
Abstract:
[Objectives] To explore volatiles of Juglans mandshurica and their effect on the brown mulberry longhorn beetle, Anoplophora
chinensis, in order to provide a theoretical basis for screening
attractants or repellents to control this
insect pest. [Methods] Volatiles
released from J. mandshurica subject to different kinds of damage
[undamaged (CK), and plants subject to feeding, oviposition and boring damage
by longicorn beetles] were collected using dynamic headspace
adsorption, and those causing electrophysiological and behavioral responses in A.
chinensis were identified by gas chromatography-mass spectrometry (GC-MS),
a gas chromatography-electrophysiological antennal detecting system (GC-EAD)
and Y-tube olfactometer experiments. [Results] A. chinensis adults had
significant electrophysiological responses to ten volatiles of J.
mandshurica, including (1R)-(+)-alpha-pinene, ocimene,
p-diethylbenzene, g-terpinene, nonanal, α-terpineol, pentamethyl phenyl,
2-ethylhexyl acrylate, 2,6-dimethylnaphthalene and hexadecane, but no response
to the other volatiles. Female and male adults had stronger
electrophysiological responses to terpenes and ocimene. The volatile (1R)-(+)-alpha-pinene
caused a significant olfactory reaction in male adults (P < 0.05), and ocimene was extremely attractive
to female and male adults (P < 0.01).
2-ethylhexyl acrylate was strongly repellent to female adults (P < 0.05) and p-diethylbenzene was highly
attractive to males (P < 0.01).
Nonanal was repellent to male adults (P < 0.05). [Conclusion] Adult female A.
chinensis are strongly attracted to ocimene and repelled by 2-ethylhexyl
acrylate. Male adults are strongly attracted to (1R)-(+)-alpha-pinene
and ocimene and repelled by p-diethylbenzene and nonanal.