[Objectives]  The Lipophorin receptor (LPR) plays an important role in lipid transport in insects. The lipophorin receptor gene of Locusta migratoria (LmLPR) was obtained from the transcriptome database, and its characteristics and expression was analyzed. RNA interference (RNAi) technology was used to explore the role of LmLPR in lipid transport. [Methods]  Based on a transcriptome database from our lab, LmLPR and its full-length Open Reading Frame (ORF) sequence were obtained and compared with the L. migratoria genomic database. Characteristics of the encoded protein sequence were analyzed after cloning and verification. The amino acid sequence of this protein was aligned with LPR amino acid sequences of other species using GENEDOC software. A phylogenetic tree of the relationship between LmLPR and homologous sequences from other species was constructed using the Neighbor-Joining method in MEGA 5.1 software. The expression pattern in different tissues of 2-day-old fifth-instar nymphs and different developmental stages were analyzed with RT-qPCR. The biological function of LmLPR was analyzed with RNAi technology and eosin staining. [Results]  Two types of L. migratoria LPR, namely a long type (LmLPR-L) and a short type (LmLPR-S), were obtained by cloning and sequence analysis. Sequence analysis revealed that both LmLPR-L and LmLPR-S have one signal peptide, four epidermal growth factor domains, five low-density lipoprotein receptor YWTD domains, and one transmembrane domain. Whereas LmLPR-L has 6 low-density lipoprotein receptor domains at the N-terminus, LmLPR-S has 7. A 38 amino acid sequence was detected at the C-terminus. The phylogenetic tree showed that LmLPR-L and LmLPR-S clustered together with the homologous BgLPR-L and BgLPR-S from Blattella germanica. The results of RT-qPCR showed that expression of LmLPR-S was high in ovarian tissue but low in other tested tissues. Expression of LmLPR-L was high in the wing pads and ovary but low in other tested tissues. The expression levels of LmLPR-S and LmLPR-L both first increased in the early stage of fifth instar, then decreased. Expression was highest on days 2 and 3 (N5D1 and N5D2), respectively. After silencing the expression of LmLPR by injecting dsLmLPR into 4th instar larvae, treated nymphs were able to complete the molt normally, as did the control group. The results of eosin staining found no evidence of significant change in cuticle permeability in both dsLmLPR and dsGFP-injected nymphs. [Conclusion]  The lipophorin receptor gene LmLPR is highly expressed in ovarian tissue, and down-regulating its expression did not affect the normal growth and development of nymphs, or the permeability of cuticle. These results provide a basis for further investigation of the role of the lipophorin receptor in the transport of insect cuticular lipids.