A method for using UPLC-MS/MS to measure β-ecdysone in Plutella xylostella
Author of the article:SUN Shi-Qing；WANG Nian-Meng；XUE Chao-Bin
Author's Workplace：College of Plant Protection, Key Laboratory of Pesticide Toxicology and Application Technique, Shandong Agricultural University, Tai’an 271018, China；College of Plant Protection, Key Laboratory of Pesticide Toxicology and Application Technique, Shandong Agricultural University, Tai’an 271018, China；College of Plant Protection, Key Laboratory of Pesticide Toxicology and Application Technique, Shandong Agricultural University, Tai’an 271018, China
Key Words：β-ecdysone; Plutella xylostella; brassinolide; internal standard method; limit of quantitation; UPLC-MS/MS
Abstract：
[Objectives] A method for using UPLC-MS/MS to detect β-ecdysone
in the serum of Plutella xylostella was developed. [Methods] Serum β-ecdysone
of P. xylostella was extracted with
acetonitrile and precipitated with protein. It was then separated in a C18 column, analyzed with UPLC-MS/MS and the β-ecdysone
content determined using the internal standard brassinolide. [Results] The results show that the linear correlation coefficient of β-ecdysone was R2 = 0.999 8 in the range of 0.5-50 μg/L, the average recovery was
91.9%-104.9% at levels of 0.5 μg/L,
10 μg/L and 50 μg/L, the relative standard deviation
(RSDs, n = 5) was 3.2%-9.2% and the quantitative limit of the method was
0.5 μg/L. [Conclusion] This method
has the advantages of being relatively simple, sensitive, stable and
applicable. It should provide an accurate method for the detection and
measurement of β-ecdysone in the
serum of other insects.