Review of insect honeydew research methods

Author of the article:ZHANG Bei-Bei；ZHANG Ze-Long；HE Hai-Fang；LI Jing-Jing；BAI Su-Fen；YAN Feng-Ming

Author's Workplace：College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China；College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China；College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China；College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China；College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China；College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China

Key Words：insect honeydew; circadian rhythms; chromogenic assay; colorimetry; honeydew-clock

Abstract：This paper summarizes the excretion rhythm, components and ecological significance of insect honeydew, and both qualitative and quantitative honeydew research methods, including chromogenic assay, weighing, colorimetry, oil drop measurement and the honeydew-clock. Detailed procedures, key points and examples of these five methods are provided, together with a comparison of their advantages and disadvantages.