Diversity of intestinal bacteria in different geographic populations of Tribolium castaneum (Coleoptera: Tenebrionidae)
Author of the article:WANG Zheng-Yan;WANG Yang;HE Meng-Ting;LU Yu-Jie
Author's Workplace:Henan University of Technology, School of Food Science and Technology;Henan University of Technology, School of Food Science and Technology;Henan University of Technology, School of Food Science and Technology;Henan University of Technology, School of Food Science and Technology
Key Words:Tribolium castaneum; gut bacteria; diversity; 16S rDNA; high-throughput sequencing
Abstract:
[Objectives] To reveal the community structure and diversity of the intestinal bacteria of Tribolium castaneum. [Methods] The Illumina MiSeq high-throughput sequencing platform was used to sequence the V3-V4 regions of the bacterial 16S rDNA gene in the intestine of adult male T. castaneum from three geographic populations. [Results] A total of 465 293 reads and 113 OTUs were obtained and annotated into 8 phyla, 15 classes, 33 orders, 52 families and 79 genera. The most common phyla were the Proteobacteria, Firmicutes, and Actinobacteria. The most abundant genera were Pseudomonas, unclassified-f-Enterobacteriaceae, Acinetobacter, Enterococcus and Rhizobium. The Shannon, Simpson, Ace and Chao indices were 1.5-1.7, 0.30, 79.7-133.2 and 76.4-85.2, respectively. [Conclusion] Bacterial community structure and diversity differ among different geographic populations of T. castaneum.