[Objectives]  To clone the full cDNA sequence of the Galeruca daurica (Coleoptera: Chrysomellidae) juvenile hormone binding protein gene, analyze its molecular characteristics and expression profiles, and provide information that will help further clarify its function in the development and summer diapause of G. daurica. [Methods]  Based on the transcriptome database of G. daurica, RACE technology was used to clone the full cDNA sequence of GdJHBP, the molecular characteristics of which were analyzed by software, including ORF Finder, SignaIP, DNAMAN and TMHMM. A neighbor- joining (NJ) phylogenetic tree was constructed using MEGA 6.0 software. Real-time quantitative PCR (RT-qPCR) was used to assay the expression profiles of GdJHBP in different developmental stages and adult tissues, and under high temperature. [Results]  The full cDNA sequence of GdJHBP (GenBank accession No.: MG460309) obtained from G. daurica was 826 bp in length with an open reading frame (ORF) 714 bp long, and encodes 237 amino acids with a predicted molecular weight of 26.58 ku and an isoelectric point of 4.37. The encoding protein is a single peptide without transmembrane structure, and contains a conserved JHBP family domain between 27 and 189 residues. Sequence alignment indicates a low amino acid sequence identity between the JHBPs of different insects; the highest identity, with Rhynchophorus ferrugineus RfJHBP and Leptinotarsa decemlineata LdJHBP 3p2, was only 30%. Phylogenetic analysis indicates that GdJHBP is most closely related to a hemolymph JHBP from R. ferrugineus. RT-qPCR indicates that GdJHBP was expressed in various developmental stages of G. daurica with the highest expression in larvae and the lowest in eggs and pupae. Expression of GdJHBP was relatively low during the summer diapause of G. daurica adults and was higher both before, and after, diapause. GdJHBP was more highly expressed in the thorax and abdomen than in the head during adult development. High temperatures (30 to 40 ℃) induced GdJHBP to be up-regulated with peak expression at 35 ℃. [Conclusion]  GdJHBP belongs to the hemolymph JHBP family and may play an important role in the development and summer diapause of G. daurica.