Objectives]  The  thrips Frankliniella occidentalis (Pergande) is the chief vector of the Tomato spotted wilt orthotospovirus (TSWV). This virus is transmitted in a cyclic and proliferative pattern, but the interactive proteins involved in this process are unclear. The G_N protein can be used as an indicator of interaction between F. occidentalis and TSWV, and  thereby provide a way to clarify the interactions between vector, virus and plant host. [Methods]  A cDNA library of the ubiquitin yeast, two-hybrid membrane system was constructed using the pPR3-N vector. Interacting proteins were annotated with the Gene Ontology (GO) pathway and their biological functions analyzed. [Results]  The capacity of the cDNA library is 3.2×10⁶. After the removal of repeat sequences, vector sequences and frameshift sequences, 74  thrips proteins were found to have interacted with TSWV G_N protein. Annotation results showed that these host factors were involved in 12 biological processes via the GO pathway, including cellular, metabolic and biological regulatory processes. [Conclusion]  A cDNA library of F. occidentalis was successfully constructed and proteins that interact with the TSWV G_N protein identified. These results provide a basis for further studies of the interaction between F. occidentalis and TSWV.