[Objectives]  To determine the profiles of three high expression glutathione-S-transferase (GST) genes in various developmental stages of Aphis. gossypii Glover, and compare the expression of these genes in different host-races of this pest. [Methods]  Full-length cDNA sequences of GSTs genes were cloned based on transcriptome data of A. gossypii specimens collected from seedling and mid-summer growth stages of cotton. The relative expression levels in different developmental stages (nymph and adult) and host-races (cucumber and cotton host-races) were investigated with real-time quantitative PCR. [Results]  Three high expression GSTs were identified and named AgoGST-s1 (GenBank accession no.: MN688789), AgoGST-d1 (GenBank accession no.: MN688790) and AgoGST-d2 (GenBank accession no.: MN688791). These 3 genes were expressed in all development stages and their temporal expression profiles all tended to increase over time. The relative expression of AgoGST-s1 gene was less in all developmental stages, whereas that of AgoGST-d1 and AgoGST-d2 was more variable. Expression of these 3 GSTs in the cucumber host-race was higher than in the cotton host-race for most age-classes. [Conclusion]  Expression of the AgoGST-s1, AgoGST-d1 and AgoGST-d2 genes is significantly associated with the developmental stage of A. gossypii. The expression patterns of these 3 GST genes in two host-races differed, which may reflect adaptation to different host plants.