[Objectives]  Guadipyr is a novel insecticide that has both neonicotinoid and semicarbazone insecticidal activity. How it affects ion channels and channel gating characteristics and functions is currently unknown. We examined the effects of Guadipyr on voltage-gated calcium and potassium channels, and explored whether these channels are potential targets for this pesticide. [Methods]  The effects of three concentrations (1, 10, and 100 μmol∙L^－1) of Guadipyr on Ca²⁺and K⁺ channels were studied using the patch clamp technique. [Results]  All three concentrations of Guadipyr significantly (P<0.05) shifted Ca²⁺ channel Ⅰ-Ⅴ and activation curves about 10-15 mV in the negative direction. The inactivation curves of Ca²⁺ channels were also shifted 5 mV towards the hyperpolarizing direction, which is a not significant shift (P>0.05). The Ca²⁺ peak current was significantly decreased by Guadipyr treatment relative to the control. The I_peak decreased more slowly as the concentration of Guadipyr increased. The area of window current increased the most (93.20%) following treatment with 1 μmol∙L^－1 Guadipyr than by treatment with 10 μmol∙L^－1 and 100 μmol∙L^－1. The number of Ca²⁺ channels activated increased after Guadipyr treatment at a test voltage of ﹣60 mV to +10 mV. The K⁺ peak current decreased gradually when Guadipyr was added to the external solution; the I_peak decreased more slowly as the Guadipyr concentration increased from 10 μmol∙L^－1 to 100 μmol∙L^－1. Ⅰ-Ⅴ curves declined and the activation of K⁺ channels was shifted 8 mV towards the depolarizing direction, which was not significant (P>0.05). This shows that K⁺ channels can be activated at higher potentials by Guadipyr. [Conclusion]  Guadipyr can effectively inhibit the I_peak of the Ca²⁺and K⁺ channels, and shift the activation and inactivation curves of these channels, thereby affecting the gating of Ca²⁺ channels and K⁺ channels. Therefore, Ca²⁺ and K⁺ channels in the central neurons of H. armigera are potential Guadipyr target sites.