[Objectives]  In order to improve understanding of the role of cytochrome P450 in the detoxification of plant secondary substances and insecticides, the CPR gene (CpCPR) of the codling moth, Cydia pomonella, was cloned and its expression profile was analyzed. [Methods]  The amino acid sequences of CPR genes from closely-related insect species were used as queries to search against the C. pomonella transcriptome database (SRX371333) and obtain the sequence of the C. pomonella CPR gene. RT-PCR was then used to amplify the Open Reading Frame (ORF) of the target gene. Bioinformatics software was used to compare the sequence characteristics, 3D structure and phylogenetic relationship of CpCPR to those of CPR from other insects. Real-time quantitative PCR (RT-qPCR) was used to determine the relative expression level of CpCPR in different developmental stages (eggs, 1-5 instar larvae, pupae, and adults) and in various tissues (head, cuticle, fat body, midgut, and Malpighian tubes). [Results]  The length of the CpCPR gene is 20 252 bp, which encodes 683 amino acids with a predicted molecular mass of 77.326 ku and a theoretical isoelectric point of 5.65. CpCPR contains typical insect CPR gene characteristics, such as an FMN region, an NADPH region and a FAD region. Phylogenetic analysis indicates that CpCPR clusters with the CPR gene of other Lepidoptera. RT-qPCR results show that the CpCPR gene is expressed in all developmental stages but is most abundant in the larval stage. In addition, CpCPR mRNA was detected in all tissues but was most abundant in the midgut in fourth-instar larvae. [Conclusion]  The ORF of the CpCPR gene was successfully cloned. This gene was most highly expressed during the larval stage and in the midgut of C. pomonella, indicating that it may play an important role in the detoxification of plant secondary substances and insecticides.