Identification and analysis of Apis cerana cerana forager antennae proteomics
Author of the article:HUANG Yu-Bin;LI Yan;DANG Xiao-Qun;ZHOU Ze-Yang
Author's Workplace:College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;College of Life Sciences, Chongqing Normal University, Chongqing 401331, China
Key Words:Apis cerana cerana; antenna protein; proteomic analysis; NPC2
Abstract:
[Objectives] To investigate
the role of the antennae in pollen collection and reproduction in bees and
thereby provide a foundation for further study of the olfactory sensory
mechanism of bees. [Methods] Antennae
were collected from Apis cerana cerana foragers and full spectrum
protein analysis used to identify the antennal proteins. LC-MS/MS was used to identify the total
antennal proteins and GO and KEGG analysis was performed on all proteins
identified with OmicsBean. Semi-quantitative
qRT-PCR was used to detect the transcription of NPC2 (Niemann-Pick proteins of type C2). [Results] 1 231 proteins were identified, including 14 olfactory-related proteins. GO and KEGG analyses indicate that most proteins are involved in
binding and catalytic activity. 297 proteins are involved in metabolic pathways
such as carbon and amino acid metabolism, TCA cycle and lipid metabolism. 43
proteins are involved in processing developmental and environmental
information, and 67 in processing genetic information. About 12% of the
proteins identified are involved in small molecule binding. qRT-PCR indicates that NPC2 was
most highly expressed in the antennae of drones. [Conclusion] The antennal proteins of foraging bees are mainly involved in
antennal development and function. The results on the function of
olfactory-related proteins
provides support for further study of odorant recognition and the olfactory
transport mechanism in A. cerana.