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Your Position :Home->Past Journals Catalog->2020年57 No.5

Identification and analysis of Apis cerana cerana forager antennae proteomics
Author of the article:HUANG Yu-Bin;LI Yan;DANG Xiao-Qun;ZHOU Ze-Yang
Author's Workplace:College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;College of Life Sciences, Chongqing Normal University, Chongqing 401331, China
Key Words:Apis cerana cerana; antenna protein; proteomic analysis; NPC2
Abstract:
[Objectives]  To investigate the role of the antennae in pollen collection and reproduction in bees and thereby provide a foundation for further study of the olfactory sensory mechanism of bees. [Methods]  Antennae were collected from Apis cerana cerana foragers and full spectrum protein analysis used to identify the antennal proteins. LC-MS/MS was used to identify the total antennal proteins and GO and KEGG analysis was performed on all proteins identified with OmicsBean. Semi-quantitative qRT-PCR was used to detect the transcription of NPC2 (Niemann-Pick proteins of type C2). [Results]  1 231 proteins were identified, including 14 olfactory-related proteins. GO and KEGG analyses indicate that most proteins are involved in binding and catalytic activity. 297 proteins are involved in metabolic pathways such as carbon and amino acid metabolism, TCA cycle and lipid metabolism. 43 proteins are involved in processing developmental and environmental information, and 67 in processing genetic information. About 12% of the proteins identified are involved in small molecule binding. qRT-PCR indicates that NPC2 was most highly expressed in the antennae of drones. [Conclusion]  The antennal proteins of foraging bees are mainly involved in antennal development and function. The results on the function of olfactory-related proteins provides support for further study of odorant recognition and the olfactory transport mechanism in A. cerana.
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