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Issue:ISSN 2095-1353
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Your Position :Home->Past Journals Catalog->2021年58 No.1

Evaluation of the stability of reference genes in adult female Mythimna separata under a near-zero magnetic field
Author of the article:YAN Meng-Meng;ZHANG Lei;CHENG Yun-Xia;JIANG Xing-Fu
Author's Workplace:State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, Chin
Key Words:near-zero magnetic field; geomagnetic field; Mythimna separata; reference gene; quantitive real-time PCR
Abstract:
[Objectives]  To identify a stable reference gene in adult Mythimna separata under a near-zero magnetic field in order to provide a way of assessing the accuracy of quantitative analysis of target gene expression in this species. [Methods] M. separata were raised under a near-zero magnetic field generated by a Helmholtz coil, with a control group raised under a geomagnetic field. 10 reference genes were chosen and evaluated,including β-actin (β-Actin), β-tubulin (β-TUB), TATA box binding protein (TBP), elongation factor (EF-1α), olealdehyde- 3-phosphate dehydrogenase (GAPDH), 18S RNA ribosome (18S rRNA), 28S ribosome RNA (28S rRNA), cGMP-dependent protein kinase (PKG), ribosomal protein L12 (RPL12) and adenosine triphosphatase (ATPase). RT-PCR technology was used to determine the expression level of these genes in newly emerged females raised under either the near-zero magnetic, or geomagnetic, fields, and four reference screening analysis software packages, geNorm, NormFinder, BestKeeper and RefFinder, were used to evaluate the stability of reference genes in each group. [Results]  β-Actin and 18S were ranked the most stable by NormFinder, and β-Actin and PKG by geNorm and BestKeeper. RefFinder analysis indicates that the most stable internal reference genes under the two different magnetic field treatments were the β-Actin18S and the PKG, genes. β-TUB and TBP were the most unstable expression reference genes. Further analysis using geNorm software indicates that it is best to use two reference genes. [Conclusion]  The most stable reference genes for adult female M. separata under different magnetic field intensities were identified, providing a foundation for the accurate quantification of the transcription of key genes involved in the magnetic reception mechanism in this species.
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