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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2021年58 No.1

Genetic diversity of geographical populations of Chilo suppressalis (Walker) in Sichuan province
Author of the article:YU Wen-Juan;CHEN Dan;LUO Han;JI Hong-Li;PENG Yun-Liang
Author's Workplace:1. Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, MOA Key Laboratory of Integrated Management of Pests on Crops in Southwest China, Chengdu 610066, China; 2. China National Rice Research Institute, National Key Laboratory of Rice Biology, Hangzhou 311401, China
Key Words:Chilo suppressalis (Walker); genetic diversity; cytochrome C oxidase subunit Ⅱ; internal transcribed spacer; geographical population
Abstract:
[Objectives]  To investigate genetic diversity among geographic populations of Chilo suppressalis in Sichuan province and thereby provide information to improve the regional management of this pest. [Methods]  The mitochondrial DNA (mtDNA) cytochrome oxidase subunit Ⅱ (COⅡ) and internal transcribed spacers (ITS) located in ribosomal DNA (rDNA) of 17 geographical Sichuan populations of C. suppressalis were sequenced and compared with MEGA. [Results]  A 500 bp segment of the COⅡ gene among 114 mature larvae was sequenced and analyzed, and 147 variable sites and 33 haplotypes identified. The haplotype diversity of different populations ranged from 0.703 83 in eastern Sichuan to 0.802 26 in western Sichuan. Phylogenetic trees showed that all geographic populations except Meishan-1-1grouped in a single great clade. Sequencing of a 533 bp ITS segment identified 299 variable sites and 92 haplotypes. Haplotype diversity among the different populations ranged from 0.967 48 in eastern Sichuan to 0.975 71 in western Sichuan. Phylogenetic trees indicate that the Qianwei population groups in a single clade whereas all other geographic populations are in another clade. [Conclusion]  Both COⅡ and ITS haplotype diversity indicate that C. suppressalis are more diverse in western Sichuan than other parts of the province. There is little correlation between genetic diversity and geographic distribution, which may reflect the fact that there was originally low divergence among populations.
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