The extraction and identification of active components of the sex pheromones of Zeuzera leuconotum Butler (Lepidoptera: Cossidae)
Author of the article:LIU Hong-Xia;JING Xiao-Yuan;ZHENG Hai-Xia;YANG Mei-Hon;ZHANG Jin-Tong;ZHU Wei-Hua;LIU Jin-Lo
Author's Workplace:Department of Basic Courses, Shanxi Agriculture University (Shanxi Academy of Agriculture Science), Taigu 030801, China; College of Life Science, Shanxi Agriculture University (Shanxi Academy of Agriculture Science), Taigu 030801, China; Colledge of Agriculture, College of Life Science, Shanxi Agriculture University (Shanxi Academy of Agriculture Science), Taigu 030801, China; Agricultural Technology Extension Service of Huairen, Huairen 035300, China
Key Words:Zeuzera leuconotum Butler; sex pheromone; identification; EAG
Abstract:
[Objectives] To identify the
active components of the sex pheromone of female Zeuzera leuconotum Butler moths. [Methods] Sex pheromones were extracted from the sex
glands of female moths with n-hexane and the active components analyzed using
gas chromatography (GC) and gas chromatography–mass spectrometry (GC-MS).
Electroantennograms (EAG) were used to examine the EAG responses of male
antennae to extracts and standard components. [Results] GC results indicate that there are 7 chemicals
in the extract, and that the peak height and area of component G increased
after the addition of standard compound E2,Z13-18:Ac. Based on GC-MS data,
component G was identified as E2,Z13-18:Ac. The EAG results show that all
compounds caused an antennal reaction, and that E2,Z13-18:Ac elicited the
strongest EAG activity. [Conclusion] E2,Z13-18:Ac was identified as the main
component of the sex pheromone of Z. leuconotum. These results provide a theoretical basis for
developing synthetic sex pheromone lures to control this pest.