Cloning and expression of the attacin gene in Gomphocerus sibiricus
Author of the article:WEI Xiao-Jia YE Xiao-Fang SUN Wen-Qing WANG Jia-Lin WANG Zhen-Hong HALIYA ? Geming JI Rong H
Author's Workplace：International Center for the Collaborative Management of Cross-border Pest in Central Asia, Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; International Center for the Collaborative Management of Cross-border Pest in Central Asia, Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; International Center for the Collaborative Management of Cross-border Pest in Central Asia, Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; International Center for the Collaborative Management of Cross-border Pest in Central Asia, Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; International Center for the Collaborative Management of Cross-border Pest in Central Asia, Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; International Center for the Collaborative Management of Cross-border Pest in Central Asia, Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; International Center for the Collaborative Management of Cross-border Pest in Central Asia, Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China; International Center for the Collaborative Management of Cross-border Pest in Central Asia, Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China
Key Words：Gomphocerus sibiricus; attacin gene; bioinformatics analysis; expression pattern
Abstract：
[Objectives] The attacin gene of Gomphocerus sibiricus was amplified and sequenced and its
differential expression in different tissues was quantified. [Methods] The attacin gene was cloned using RACE technology and its primary structure, secondary
structure, tertiary structure, phylogeny and subcellular localization, analyzed
with bioinformatics software. Real-time quantitative PCR(RT-qPCR) was used to
detect the relative expression of the gene in the Malpighian tubules, midgut,
hindgut, fat body and salivary glands. [Results] The cloned attacin gene contained an open reading frame of 282 bp and encoded 93 amino acid
residues. Its predicted molecular weights is 9.98 ku, and its isoelectric point
is 9.26. Sequencing revealed signal peptide sequences. The gene was most highly
expressed in the midgut. [Conclusion] Differences
in expression levels of the attacin gene in different adult tissues of G. sibiricus suggests
that the digestive tract plays an important role in preventing the invasion of
pathogenic microorganisms.