Stress response of Tetranychus truncatus to pyridaben and high temperatures
Author of the article:FU Wen-Hua QIAO Wan-Qiang ZHANG Cui-Wen ZHANG Shuang-Hong WANG Ji-Zu YU Cai-Lan WANG Sen-Shan
Author's Workplace：College of Plant of Gansu Agricultural University, Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China; College of Plant of Gansu Agricultural University, Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China; College of Plant of Gansu Agricultural University, Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China; College of Plant of Gansu Agricultural University, Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China; College of Plant of Gansu Agricultural University, Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China; College of Plant of Gansu Agricultural University, Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China; College of Plant of Gansu Agricultural University, Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China; College of Plant of Gansu Agricultural University, Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China
Key Words：pyridaben; high temperature; Tetranychus truncatus; free radicals; protective enzyme
Abstract：
[Objectives] To investigate
changes in free radical and protective enzyme levels induced by exposure to
pyridaben and a high temperature, in a
sensitive (SS) and pyryl-R resistant (Py-R) strain of Tetranychus truncatus. [Methods] Soluble protein content and activity of
the superoxide radical (∙O2－), superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), in
the SS and Py-R strains were determined after treatment with pyridaben and
exposure to 38 ℃. The interaction between free radicals and
three protective enzymes was also analyzed. [Results] The ∙O2－ content of the SS
and Py-R strains were 5.5192 and 1.3462 times higher than that of the control
group after exposure to pyridaben and the high temperature, and SOD activity was
5.344 7 and 7.105 2 times higher. CAT and POD activity were also higher than in
the control group. [Conclusion] ∙O2－, SOD, CAT and POD levels in T.
truncatus are affected by pyridaben and high temperature stress, and the
Py-R strain has higher protective enzyme activity under long-term pyridaben
stress.