Latest Cover

Online Office

Contact Us

Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Tel:+86-10-64807137
Fax:+86-10-64807137
Email:entom@ioz.ac.cn
Your Position :Home->Past Journals Catalog->2021年58 No.5

Electron microscopic observation of infection of Adelphocoris suturalis by Beauveria bassiana
Author of the article:ZHANG Xiao LIU Jia YANG Yi-Hua WU Shu-Wen
Author's Workplace:College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
Key Words:Beauveria bassiana; Adelphocoris suturalis; scanning electron microscopy; transmission electron microscopy; infection process
Abstract:
[Objectives]  To reveal the pathogenic mechanism of infection of Beauveria bassiana and investigate the effectiveness of B. bassiana as a biological control for mirid bugs. [Methods]  The process of infection of Adelphocoris suturalis by the Bb-VIII strain of B. bassiana was observed with both scanning and transmission electron microscopy. [Results]  At the beginning of inoculation, B. bassiana adhered to epidermal folds, depressions and body joints of A. suturalis. After 16 h germinated conidia formed germ tubes that invaded the cuticle of A. suturalis with the aid of extracellular hydrolyzing enzymes and mechanical stress. After 36-72 h hyphal bodies had invaded muscle tissue, the fat body, intestinal villi, proliferating in the body cavity by both septation and budding. Finally, after 48 h hyphae emerged from the cuticle and developed into aerial hyphae and conidia. The cuticle of dead A. suturalis was completely covered by hyphae. [Conclusion]  Attachment of B. bassiana conidia is affected by structural features of the body wall of A. suturalis. The infection process was completed within 48 h of inoculation, which shows that B. bassiana is highly pathogenic to A. suturalis. The mechanism by which hyphae invade the host cuticle involves not only mechanical pressure but also extracellular hydrolase.
CopyRight©2024 Chinese Journal of Aplied Entomology