Effects of plant secondary metabolites on the growth, development and detoxification enzyme activity, of Spodoptera exigua
Author of the article:FAN Neng-Neng;WANG Jin-Yan;WAN Nian-Feng;JIANG Jie-Xian
Author's Workplace:College of Life Science and Fishery, Shanghai Ocean University, Shanghai 201306, China; Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Key Words:quercetin; kaempferol; coumarin; mortality; caboxylesterase; glutathione -S-transferase; cytochrome P450
Abstract:
[Objectives] To determine
the effects of plant secondary metabolites on the growth, development and
detoxification enzyme activity, of Spodoptera
exigua, and investigate the potential of using these substances to control
this pest. [Methods] Three different concentrations
(0.01%, 0.1% and 1.0%) of three different plant secondary compounds, quercetin,
kaempferol or coumarin, were added to the artificial diet fed to 3rd instar S. exigua larvae and their growth and development were observed
and compared. In addition, the activity of caboxylesterase, glutathione S-transferase and cytochrome P450, were
measured 24, 48 and 72 h after larvae had been treated with 0.1% quercetin,
kaempferol or coumarin. [Results] All three secondary metabolites significantly increased
larval mortality. The pupal weight of larvae treated with of 1.0% quercetin was
significantly less than that of the control group, whereas their developmental
duration was obviously longer. CarE activity was significantly higher in larvae
treated with 0.1% kaempferol, but 0.1% quercetin and 0.1% coumarin
significantly inhibited CarE activity. Although quercetin had no significant
effect on GSTs, 0.1% kaempferol and 0.1% coumarin increased GSTs. The P450
activity of larvae treated with 0.1% quercetin or 0.1% coumarin was higher than
that of the control group, although not significantly so, whereas the P450
activity of larvae treated with 0.1% kaempferol was significantly lower than
that of the control group after 48 hours. [Conclusion] Different plant secondary metabolites have
different effects on the growth and detoxification enzyme activity of S.
exigua larvae.