Varroa destructor is an ectoparasitic mite causing devastating damages to Apis mellifera. The long-term, widespread and continuous use of acaricides result in the acaricide resistance of mite, which becomes more and more serious. Overcome the resistance of Varroa destructor makes a great significance for mite prevention and control. The main mechanism of acaricide resistance is target insensivity and detoxification enhancement, and is affected by genetic factors, artificial operation factors and other ecological factors, which is much clear of pyrethroids, but researches on organophosphorus and formamidines are still lacking. This paper described the research progress on the resistance mechanism of Varroa mite to pyrethroids, organophosphates and formamidines, discussed the latest results of resistance management and future research directions in this field, which will provide reference for the effective management of Varroa mite, for the suitable use of acaricide and development of new acaricides.