The application of Ectropis obliqua nucleopolyhedroviruses preparations as a biological control for Ectropis grisescens in the field
Author of the article:TANG Mei-Jun GUO Hua-Wei LI Hong ZHANG Xin-Xin WANG Zhi-Bo YAO Hui-Ming ZHOU Xiao-Gui XIAO Q
Author's Workplace： Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China; Graduate School of the Chinese Academy of Agricultural Sciences, Beijing 100081, China
Key Words：nucleopolyhedrovirus; Ectropis grisescens; application technology
Abstract：
[Objectives] To investigate techniques for using Ectropis obliqua nucleopolyhedrovirus (EcobNPV) preparations, a pathogen that can infect both Ectropis obliqua and Ectropis grisescens, to control these pests. [Methods] The effects of different application methods (drone and sprayer）, larval instar (1st, 2nd and 3rd instar larvae) and dosages (75, 150 and 375 mL/667 m2) on the effectiveness of EcobNPV preparations containing both 1×104 PIB/μL EcobNPV and 2 000 IU/μLBacillus thuringensis, were tested in field trials. [Results] There was no significant difference in the effectiveness of EcobNPV preparations of the same dosage applied with either a drone (69.0%) or sprayer (67.6%). The optimal dose of EcobNPV preparations was 375 mL/667 m2, followed by 150 mL/667 m2; the effectiveness of both doses exceeded 90%. The effectiveness of EcobNPV preparations on 1st, 2nd and 3rd instar larvae was 93.3%, 78.7% and 67.8%, respectively, and its effectiveness on 1st instar larvae was significantly higher than that on 3rd instar larvae. [Conclusion] EcobNPV preparations can be applied by drones to control tea geometrids. First instar larvae are most susceptible to EcobNPV preparations and the optimal dose is 150-375 mL/667 m2.