Roles of the chemosensory protein NlugCSP6 gene in the development, reproduction and wing formation of Nilaparvata lugens
Author of the article:GAO Hao-Li;ZOU Jian-Zheng;LIN Xu-Min;ZHANG Hui-Hui;LIU Ze-Wen
Author's Workplace:College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
Key Words:Nilaparvata lugens; chemonsensory proteins; NlugCSP6; development; reproduction; wing development
Abstract:
[Objectives] To investigate the
potential non-chemosensory functions of NlugCSP6, a chemosensory protein
(CSP) gene with high expression levels in the epidermis and ovary of Nilaparvata lugens (Stål). [Methods] The development and tissue expression profile of NlugCSP6 were determined using RT-qPCR,
and the role of NlugCSP6 in nymph development, reproduction and wing formation
investigated using RNAi. [Results] NlugCSP6 was expressed in all
developmental stages with the highest expression in the epidermis, followed by
the ovary. Knockdown of NlugCSP6 significantly decreased nymph survival
and reduced egg production aid by 22%. Knockdown also caused abnormal wing
development; deformed female wings and ineffective wing closure in males. [Conclusion] The NlugCSP6 gene is highly expressed in the epidermis, and
is essential for normal nymph development and wing formation in N. lugens.
Its relatively high expression in the ovary suggests that the NlugCSP6 gene is also important for female reproduction. These results indicate that the
role of the NlugCSP6 gene in the development and reproduction of N.
lugens is consistent with its degree of expression in different tissues.