Spatiotemporal expression of CYP6ER1 and response to dinotefuran stress in Nilaparvata lugens
Author of the article:XIAO Han-Xiang；LI Yan-Fang；CHEN Shan-Yuan；DAI Yang-Shuo；YUAN Long-Yu；ZHANG Zhen-Fei
Author's Workplace：Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China
Key Words：Nilaparvata lugens; pesticide resistance; CYP6ER1; differential expression
Abstract：
[Objectives] To investigate the molecular
mechanism responsible for the resistance of Nilaparvata
lugens to dinotefuran. [Methods] The expression of the CYP6ER1 gene and the activity of P450 enzymes were measured and
compared in brown planthopper (BPH) populations with different levels of
resistance to dinotefuran. The expression of the CYP6ER1 gene in different developmental stages and tissues and the
activity of P450 enzymes, were measured after treatment with semi-lethal
concentrations of dinotefuran. Susceptibility to dinotefuran was tested using
the stem-dip method in a laboratory. [Results] The CYP6ER1 gene was significantly more highly expressed in resistant than in sensitive,
populations. It was also more highly expressed in 3 to 9 day-old adults than in
other age groups, and in midgut tissue than in other tissues. BPHs treated with
semi-lethal concentrations of dinotefuran had significantly different CYP6ER1 gene expression and P450 enzyme
activity to the control group. [Conclusion] There is a correlation between expression of
the CYP6ER1 gene and resistance in
different N. lugens populations. CYP6ER1 may therefore be involved in the
detoxification of dinotefuran in the brown planthopper.