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Your Position :Home->Past Journals Catalog->2009年46 No.2

The relationship between the heat shock protein 90 and the development of Microplitis mediator
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Key Words:Microplitis mediator, Hsp90, diapause, RACE, semiquantitative RT-PCR
Abstract:The heat shock protein 90 gene (hsp90) from Microplitis mediator Haliday was isolated through PCR, RACE and confirmed by semiquantitative PCR. The results revealed that both heat shock and cold shock could induce the expression of Hsp90, no matter the larvae were under the condition of diapause or not; The highest expression level appeared at the early stage of the 2ndinstar larvae, whereas the expression level decreased with development, the expression of Hsp90 increased after the adult eclosion. During the maintenance period under 4℃, the expression of Hsp90 showed significant increment at the initial days but the level declined with the elongation of maintenance time. 
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