The effect of three kinds of photoperiods (L8∶D16, L12∶D12, L16∶D8) on 4th instar nymph of three grasshopper species were investigated in Inner Mongolia grassland. The results showed that under the condition of (28±1)℃ during the day time and (23±1)℃ at night, photoperiods had little effect on the development, eclosion, and reproduction of Dasyhippus barbipes (F.-W.) and Oedaleus decorus asiaticus B.-Bidenko, but influenced the survival of these two species of grasshoppers significantly (Ｐ<0.01). D. barbipes and O. d. asiaticus had the fastest developmental rate at middle photoperiod (L12∶D12). Short photoperiod (L8∶D16) was beneficial to development of Chorthippus fallax (Zub.), followed by middle photoperiod. D. barbipes had the highest eclosing rate at middle photoperiod, but long photoperiods (L16∶D8) were beneficial to that of O. d. asiaticus and C. fallax. However, photoperiods had significant effect on oviposition ability of these three species. Middle and long photoperiod were beneficial to oviposition of D. barbipes and O. d. asiaticus, while short and middle photoperiod were beneficial to oviposition of C. falla. This phenomena was matched with the phenology of these three grasshopper species.