Phylogenetic analysis of eight species of Pimeliinae based on COⅡ and Cytbgene sequences
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Key Words:Pimeliinae, Cytb gene, COⅡ gene, phylogeny
Abstract:The phylogenetic relationships among 8 species of Pimeliinae based on a 579 bp Cytb and a 585 bp COⅡ gene sequences were analyzed. The results showed that the average contents of T, C, A and G in the 1 164 bp sequences were 33.7%, 21.2%, 33.5 and 11.6%, respectively. The content of A+T is significantly higher than that of C+G, with 77.2% of A+T at the third codon position but only 4.0% of G at the same position. Transition/transversion mostly occur between CT or AT at the third position. With Eumylada potanini as outgroup, phylogenetic trees were reconstructed based on these DNA sequences. The trees showed that Platyopini and Pimeliini were placed in a clade, which is consistent with the current opinion that Platyopini was merged into Pimeliini. Meanwhile, Tentyriini formed another monophyletic clade in the tree.