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Your Position :Home->Past Journals Catalog->2008年45 No.2

Insecticidal activities of metabolites from two Paecilomyces spp. to the small cabbage white, Pieris rapae
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Key Words:Paecilomyces spp., metabolites, insecticidal activity, Pieris rapae
Abstract:

The insecticidal activities of metabolites produced by Paecilomyces lilacinus and Paeecilomyces sp.X1 strain isolated from the infected aphids, against the small cabbage white, Pieris rapae (L.) (Lepidoptera: Pieridae) were reported in this paper. The methanol fractions were separated in the extensive separation by silica gel column and the subsequent biological activity tests showed that the 6th fraction of P.lilacinus(DM6) and the 5th fraction of Pieris sp. X1(X1M5) were their active fractions. The biological activity for P. rapae 4th instar larvae was investigated and the results showed that the AFC50 of DM6 and X1M5

were 5.865 2 mg/mL and 3.523 9 mg/mL in choice test and 9.009 5 mg/mLand 14.023 4 mg/mL in nochoice test after treatment 24 h, respectively. The active fractions DM6 and X1M5 may possessed strong toxicity against P. rapae 4th instar larvae by feeding, injection and topical application method, for example the LD50 of DM6 and X1M5 were 0.184 6 μg/larva and 0.678 4 μg/larva after treatment 72 h by injection method. The results showed the development inhibited rate (DIR) for 3rd instar larvae was 73.34% and 63.30% for DM6 and X1M5 respectively, by feeding contaminated leaves on the concentration of 5.0 mg/mL.

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